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Image Search Results
Journal: Journal of Cancer
Article Title: Comprehensive analysis based on glycolytic and glutaminolytic pathways signature for predicting prognosis and immunotherapy in ovarian cancer
doi: 10.7150/jca.88359
Figure Lengend Snippet: AAK1 promotes OV progression via Notch3 pathway in vitro . ( A ) Representative immunohistochemical staining images of AAK1 in normal ovary and OV tissue from The Human Protein Atlas (THPA) database. ( B-C ) Relative expression of AAK1 between IOSE-80 cell lines and five OV line were tested by RT-qPCR ( B ) (n = 3) and western blot ( C ) (n = 3), respectively. ( D ) Immunofluorescence staining showing the subcellular localization of AAK1 protein in A2780 and OVCAR3 cells (Scale bar: 10 µm). ( E-G ) CCK8 assay ( E ), Edu assays ( F, Scale bar: 100 µm), and invasion assays ( G ) revealing the cell viability, proliferation, and invasion of A2780 and OVCAR3 cells stably transfected as indicated, respectively (n = 3). ( H ) Western blotting showing the protein levels of AAK1, Notch3, MMP-2, and MMP-9 in A2780 and OVCAR3 cells stably transfected as indicated, respectively (n = 3). * P < 0.05, ** P < 0.01, *** P < 0.001.
Article Snippet: Western blotting assay was executed as previously reported , with
Techniques: In Vitro, Immunohistochemical staining, Staining, Expressing, Quantitative RT-PCR, Western Blot, Immunofluorescence, CCK-8 Assay, Stable Transfection, Transfection
Journal: Journal of Cancer
Article Title: Comprehensive analysis based on glycolytic and glutaminolytic pathways signature for predicting prognosis and immunotherapy in ovarian cancer
doi: 10.7150/jca.88359
Figure Lengend Snippet: AAK1 promotes glutamine metabolism of OV via Notch pathway in A2780 cells. ( A-E ) The cellular Glu ( A ), α-KG ( B ), ATP ( C ), GSH ( D ), and ROS levels (E, Scale bar: 100 µm) of A2780 cells stably transfected as indicated (n = 3). (F) Western blot indicating the protein levels of AAK1, Notch3, and GLS in A2780 and OVCAR3 cells stably transfected as indicated (n = 3). ( G ) Schematic diagram of AAK1 promots glutamine metabolism and malignant progression through the Notch3 pathway in ovarian cancer. * P < 0.05, ** P < 0.01, *** P < 0.001.
Article Snippet: Western blotting assay was executed as previously reported , with
Techniques: Stable Transfection, Transfection, Western Blot
Journal: Journal of Cancer
Article Title: Comprehensive analysis based on glycolytic and glutaminolytic pathways signature for predicting prognosis and immunotherapy in ovarian cancer
doi: 10.7150/jca.88359
Figure Lengend Snippet: Therapeutic knockdown of AAK1 suppresses OV progression and metastasis in vivo . ( A ) The representative images, ( B ) tumor growth, ( C ) tumor weight, ( D ) the representative IHC images and statistical analysis results of AAK1, Notch3, GLS, MMP-2, MMP-9, and Ki-67 of xenograft tumors established by subcutaneous injection of OVCAR3 cells that were stably transfected as indicated, respectively, respectively (n = 5 per group). Scale bar: 50 μm. ( E ) The lung metastasis images (left panel) and metastatic counts (right panel) of tumor lung metastasis assay formed by tail vein injection of OVCAR3 cells that were stably transfected as indicated, respectively (n = 5 per group). Scale bar: 100 μm. ( G ) K-M curves were employed to display the survival of nude mice with distinct treatments in the tumor lung metastasis assay. * P < 0.05, ** P < 0.01, *** P < 0.001.
Article Snippet: Western blotting assay was executed as previously reported , with
Techniques: Knockdown, In Vivo, Injection, Stable Transfection, Transfection